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ihc cd163 proteintech  (Proteintech)


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    Proteintech ihc cd163 proteintech
    Ihc Cd163 Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 347 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ihc+cd163+proteintech/CD163+Antibody/pm41548046-54-8-10
    Average 96 stars, based on 347 article reviews
    ihc cd163 proteintech - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Tumor-Associated Macrophage-Derived CXCL1 Promotes Endometrial Cancer Progression Through the CXCR2/NF-κB Pathway.
    Article Snippet: Subsequently, recombinant human CXCL1 (rCXCL1; MedChemExpress, USA), CXCL1 neutralizing antibody (CXCL1- Nab; R&D Systems, USA), or the NF- κB inhibitor BAY 11- 7082 (MedChemExpress, USA) was introduced into the TABLE 2 | Antibodies used in this study. .. Application Antibody Source Catalog number Clone number Dilution IHC CD163 Proteintech 16646- 1- AP 9332 1:2000 CXCL1 Proteintech 12335- 1- AP 2919 1:200 CXCR2 Proteintech 20634- 1- AP 3579 1:200 E- cadherin Proteintech 20874- 1- AP 999 1:10000 Vimentin Proteintech 10366- 1- AP 7431 1:5000 Phospho- NF- κB p65 Proteintech 82335- 1- RR 5970 1:200 IF CXCL1 Proteintech 12335- 1- AP 2919 1:50 CXCR2 Proteintech 20634- 1- AP 3579 1:50 CD163 Proteintech 16646- 1- AP 9332 1:500 E- cadherin Proteintech 20874- 1- AP 999 1:500 NF- κB p65 Proteintech 10745- 1- AP 5970 1:200 WB CD68 Abcam AB955 KP1 1:1000 CD163 Proteintech 16646- 1- AP 9332 1:1000 CD206 Proteintech 18704- 1- AP 4360 1:500 IL10 Abways CY6705 25A3 1:1000 E- cadherin Proteintech 20874- 1- AP 999 1:50000 Vimentin Proteintech 10366- 1- AP 7431 1:6000 N- cadherin Proteintech 22018- 1- AP 1000 1:6000 CXCR2 Proteintech 20634- 1- AP 3579 1:2500 Phospho- NF- κB p65 Cell signaling technology #3033 93H1 1:1000 NF- κB p65 Proteintech 10745- 1- AP 5970 1:3000 Phospho- IκBα Cell signaling technology #2859 14D4 1:1000 IκBα Proteintech 10268- 1- AP 4792 1:20000 Phospho- IKK α/β Cell signaling technology #2697 16A6 1:1000 IKKα Zenbio R24674 1147 1:1000 IKKβ Zenbio R24677 3553 1:1000 Anti- beta Actin Proteintech 66009- 1- Ig 60 1:20000 GAPDH Proteintech 60004- 1- Ig 2597 1:20000 Abbreviations: IF, immunofluorescence; IHC, immunohistochemical; WB, Western blot. ..

    Western Blot:

    Article Title: Tumor-Associated Macrophage-Derived CXCL1 Promotes Endometrial Cancer Progression Through the CXCR2/NF-κB Pathway.
    Article Snippet: Subsequently, recombinant human CXCL1 (rCXCL1; MedChemExpress, USA), CXCL1 neutralizing antibody (CXCL1- Nab; R&D Systems, USA), or the NF- κB inhibitor BAY 11- 7082 (MedChemExpress, USA) was introduced into the TABLE 2 | Antibodies used in this study. .. Application Antibody Source Catalog number Clone number Dilution IHC CD163 Proteintech 16646- 1- AP 9332 1:2000 CXCL1 Proteintech 12335- 1- AP 2919 1:200 CXCR2 Proteintech 20634- 1- AP 3579 1:200 E- cadherin Proteintech 20874- 1- AP 999 1:10000 Vimentin Proteintech 10366- 1- AP 7431 1:5000 Phospho- NF- κB p65 Proteintech 82335- 1- RR 5970 1:200 IF CXCL1 Proteintech 12335- 1- AP 2919 1:50 CXCR2 Proteintech 20634- 1- AP 3579 1:50 CD163 Proteintech 16646- 1- AP 9332 1:500 E- cadherin Proteintech 20874- 1- AP 999 1:500 NF- κB p65 Proteintech 10745- 1- AP 5970 1:200 WB CD68 Abcam AB955 KP1 1:1000 CD163 Proteintech 16646- 1- AP 9332 1:1000 CD206 Proteintech 18704- 1- AP 4360 1:500 IL10 Abways CY6705 25A3 1:1000 E- cadherin Proteintech 20874- 1- AP 999 1:50000 Vimentin Proteintech 10366- 1- AP 7431 1:6000 N- cadherin Proteintech 22018- 1- AP 1000 1:6000 CXCR2 Proteintech 20634- 1- AP 3579 1:2500 Phospho- NF- κB p65 Cell signaling technology #3033 93H1 1:1000 NF- κB p65 Proteintech 10745- 1- AP 5970 1:3000 Phospho- IκBα Cell signaling technology #2859 14D4 1:1000 IκBα Proteintech 10268- 1- AP 4792 1:20000 Phospho- IKK α/β Cell signaling technology #2697 16A6 1:1000 IKKα Zenbio R24674 1147 1:1000 IKKβ Zenbio R24677 3553 1:1000 Anti- beta Actin Proteintech 66009- 1- Ig 60 1:20000 GAPDH Proteintech 60004- 1- Ig 2597 1:20000 Abbreviations: IF, immunofluorescence; IHC, immunohistochemical; WB, Western blot. ..

    Immunofluorescence:

    Article Title: Tumor-Associated Macrophage-Derived CXCL1 Promotes Endometrial Cancer Progression Through the CXCR2/NF-κB Pathway.
    Article Snippet: Subsequently, recombinant human CXCL1 (rCXCL1; MedChemExpress, USA), CXCL1 neutralizing antibody (CXCL1- Nab; R&D Systems, USA), or the NF- κB inhibitor BAY 11- 7082 (MedChemExpress, USA) was introduced into the TABLE 2 | Antibodies used in this study. .. Application Antibody Source Catalog number Clone number Dilution IHC CD163 Proteintech 16646- 1- AP 9332 1:2000 CXCL1 Proteintech 12335- 1- AP 2919 1:200 CXCR2 Proteintech 20634- 1- AP 3579 1:200 E- cadherin Proteintech 20874- 1- AP 999 1:10000 Vimentin Proteintech 10366- 1- AP 7431 1:5000 Phospho- NF- κB p65 Proteintech 82335- 1- RR 5970 1:200 IF CXCL1 Proteintech 12335- 1- AP 2919 1:50 CXCR2 Proteintech 20634- 1- AP 3579 1:50 CD163 Proteintech 16646- 1- AP 9332 1:500 E- cadherin Proteintech 20874- 1- AP 999 1:500 NF- κB p65 Proteintech 10745- 1- AP 5970 1:200 WB CD68 Abcam AB955 KP1 1:1000 CD163 Proteintech 16646- 1- AP 9332 1:1000 CD206 Proteintech 18704- 1- AP 4360 1:500 IL10 Abways CY6705 25A3 1:1000 E- cadherin Proteintech 20874- 1- AP 999 1:50000 Vimentin Proteintech 10366- 1- AP 7431 1:6000 N- cadherin Proteintech 22018- 1- AP 1000 1:6000 CXCR2 Proteintech 20634- 1- AP 3579 1:2500 Phospho- NF- κB p65 Cell signaling technology #3033 93H1 1:1000 NF- κB p65 Proteintech 10745- 1- AP 5970 1:3000 Phospho- IκBα Cell signaling technology #2859 14D4 1:1000 IκBα Proteintech 10268- 1- AP 4792 1:20000 Phospho- IKK α/β Cell signaling technology #2697 16A6 1:1000 IKKα Zenbio R24674 1147 1:1000 IKKβ Zenbio R24677 3553 1:1000 Anti- beta Actin Proteintech 66009- 1- Ig 60 1:20000 GAPDH Proteintech 60004- 1- Ig 2597 1:20000 Abbreviations: IF, immunofluorescence; IHC, immunohistochemical; WB, Western blot. ..

    Immunohistochemical staining:

    Article Title: Tumor-Associated Macrophage-Derived CXCL1 Promotes Endometrial Cancer Progression Through the CXCR2/NF-κB Pathway.
    Article Snippet: Subsequently, recombinant human CXCL1 (rCXCL1; MedChemExpress, USA), CXCL1 neutralizing antibody (CXCL1- Nab; R&D Systems, USA), or the NF- κB inhibitor BAY 11- 7082 (MedChemExpress, USA) was introduced into the TABLE 2 | Antibodies used in this study. .. Application Antibody Source Catalog number Clone number Dilution IHC CD163 Proteintech 16646- 1- AP 9332 1:2000 CXCL1 Proteintech 12335- 1- AP 2919 1:200 CXCR2 Proteintech 20634- 1- AP 3579 1:200 E- cadherin Proteintech 20874- 1- AP 999 1:10000 Vimentin Proteintech 10366- 1- AP 7431 1:5000 Phospho- NF- κB p65 Proteintech 82335- 1- RR 5970 1:200 IF CXCL1 Proteintech 12335- 1- AP 2919 1:50 CXCR2 Proteintech 20634- 1- AP 3579 1:50 CD163 Proteintech 16646- 1- AP 9332 1:500 E- cadherin Proteintech 20874- 1- AP 999 1:500 NF- κB p65 Proteintech 10745- 1- AP 5970 1:200 WB CD68 Abcam AB955 KP1 1:1000 CD163 Proteintech 16646- 1- AP 9332 1:1000 CD206 Proteintech 18704- 1- AP 4360 1:500 IL10 Abways CY6705 25A3 1:1000 E- cadherin Proteintech 20874- 1- AP 999 1:50000 Vimentin Proteintech 10366- 1- AP 7431 1:6000 N- cadherin Proteintech 22018- 1- AP 1000 1:6000 CXCR2 Proteintech 20634- 1- AP 3579 1:2500 Phospho- NF- κB p65 Cell signaling technology #3033 93H1 1:1000 NF- κB p65 Proteintech 10745- 1- AP 5970 1:3000 Phospho- IκBα Cell signaling technology #2859 14D4 1:1000 IκBα Proteintech 10268- 1- AP 4792 1:20000 Phospho- IKK α/β Cell signaling technology #2697 16A6 1:1000 IKKα Zenbio R24674 1147 1:1000 IKKβ Zenbio R24677 3553 1:1000 Anti- beta Actin Proteintech 66009- 1- Ig 60 1:20000 GAPDH Proteintech 60004- 1- Ig 2597 1:20000 Abbreviations: IF, immunofluorescence; IHC, immunohistochemical; WB, Western blot. ..



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    ihc cd163 proteintech  (Proteintech)
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    Proteintech ihc cd163 proteintech
    Ihc Cd163 Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ihc+cd163+proteintech/CD163+Antibody/pm41548046-54-8-10
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    proteintech 16646 1 ap angptl4 ihc  (Proteintech)
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    Proteintech proteintech 16646 1 ap angptl4 ihc
    Fig. 2. <t>ANGPTL4</t> expression in the liver following transplantation. (A) RT-qPCR evaluation of ANGPTL4 levels in rat liver tissues. (B) ELISA results of serum <t>ANGPTL4</t> <t>protein</t> levels. (C) Immunohistochemical staining of ANGPTL4 in liver graft sections (200x magnification). ns P > 0.05, *P < 0.05; **P < 0.01; ***P < 0.001; n = 6.
    Proteintech 16646 1 Ap Angptl4 Ihc, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    proteintech 16646 1 ap angptl4 ihc - by Bioz Stars, 2026-09
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    Image Search Results


    Fig. 2. ANGPTL4 expression in the liver following transplantation. (A) RT-qPCR evaluation of ANGPTL4 levels in rat liver tissues. (B) ELISA results of serum ANGPTL4 protein levels. (C) Immunohistochemical staining of ANGPTL4 in liver graft sections (200x magnification). ns P > 0.05, *P < 0.05; **P < 0.01; ***P < 0.001; n = 6.

    Journal: Scientific reports

    Article Title: ANGPTL4 induces Kupffer cell M2 polarization to mitigate acute rejection in liver transplantation.

    doi: 10.1038/s41598-024-81832-x

    Figure Lengend Snippet: Fig. 2. ANGPTL4 expression in the liver following transplantation. (A) RT-qPCR evaluation of ANGPTL4 levels in rat liver tissues. (B) ELISA results of serum ANGPTL4 protein levels. (C) Immunohistochemical staining of ANGPTL4 in liver graft sections (200x magnification). ns P > 0.05, *P < 0.05; **P < 0.01; ***P < 0.001; n = 6.

    Article Snippet: Comparisons between two groups were made using an unpaired Student’s t-test, Name Dilution Supplier Code GAPDH WB: 1:10000 Proteintech 60004-1-Ig Arg1 WB: 1:1000 Proteintech 16001-1-AP CD86 WB: 1:1000, IHC: 1:1000 Zenbio 380,350 P65 WB: 1:1000 Proteintech 66535-1-Ig p-P65 WB: 1:1000, IF: 1:500 CST 3033 IKKβ WB: 1:1000 ABclonal A2087 p-IKKβ WB: 1:1000 Proteintech A2087 IκBα WB: 1:1000 ABclonal A19714 p-IκBα WB: 1:1000 ABclonal AP0707 Goat anti-rabbit WB: 1:20000 Zenbio 550,018 CD68 IF: 1:100 Invitrogen MA5-13324 CD163 IHC: 1:1000 Proteintech 16646-1-AP ANGPTL4 IHC: 1:1000 Proteintech 18374-1-AP Table 2.

    Techniques: Expressing, Transplantation Assay, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Staining

    Fig. 3. ANGPTL4 recombinant protein mitigates AR following liver transplantation. (A, B) Representative HE images of liver grafts taken 7 days post-transplantation at original magnifications of ×100 and ×400, along with RAI scoring of the liver. (C) TUNEL staining to identify apoptotic cells in liver tissues across different groups post-transplantation (200× magnification). (D) Measurement of serum liver enzyme levels after transplantation using a microtiter assay. (E) ELISA evaluation of serum inflammatory factors in rats following liver transplantation. ns P > 0.05, *P < 0.05; **P < 0.01; ***P < 0.001; n = 6.

    Journal: Scientific reports

    Article Title: ANGPTL4 induces Kupffer cell M2 polarization to mitigate acute rejection in liver transplantation.

    doi: 10.1038/s41598-024-81832-x

    Figure Lengend Snippet: Fig. 3. ANGPTL4 recombinant protein mitigates AR following liver transplantation. (A, B) Representative HE images of liver grafts taken 7 days post-transplantation at original magnifications of ×100 and ×400, along with RAI scoring of the liver. (C) TUNEL staining to identify apoptotic cells in liver tissues across different groups post-transplantation (200× magnification). (D) Measurement of serum liver enzyme levels after transplantation using a microtiter assay. (E) ELISA evaluation of serum inflammatory factors in rats following liver transplantation. ns P > 0.05, *P < 0.05; **P < 0.01; ***P < 0.001; n = 6.

    Article Snippet: Comparisons between two groups were made using an unpaired Student’s t-test, Name Dilution Supplier Code GAPDH WB: 1:10000 Proteintech 60004-1-Ig Arg1 WB: 1:1000 Proteintech 16001-1-AP CD86 WB: 1:1000, IHC: 1:1000 Zenbio 380,350 P65 WB: 1:1000 Proteintech 66535-1-Ig p-P65 WB: 1:1000, IF: 1:500 CST 3033 IKKβ WB: 1:1000 ABclonal A2087 p-IKKβ WB: 1:1000 Proteintech A2087 IκBα WB: 1:1000 ABclonal A19714 p-IκBα WB: 1:1000 ABclonal AP0707 Goat anti-rabbit WB: 1:20000 Zenbio 550,018 CD68 IF: 1:100 Invitrogen MA5-13324 CD163 IHC: 1:1000 Proteintech 16646-1-AP ANGPTL4 IHC: 1:1000 Proteintech 18374-1-AP Table 2.

    Techniques: Recombinant, Transplantation Assay, TUNEL Assay, Staining, Enzyme-linked Immunosorbent Assay

    Fig. 4. ANGPTL4 facilitates the in vivo shift of KCs towards the M2 phenotype. (A) WB analysis of polarization-related protein expression in KCs, with quantitative analysis based on band intensity. (B) Immunohistochemical staining of CD86 and CD163. ns P > 0.05, *P < 0.05; **P < 0.01; ***P < 0.001; n = 6.

    Journal: Scientific reports

    Article Title: ANGPTL4 induces Kupffer cell M2 polarization to mitigate acute rejection in liver transplantation.

    doi: 10.1038/s41598-024-81832-x

    Figure Lengend Snippet: Fig. 4. ANGPTL4 facilitates the in vivo shift of KCs towards the M2 phenotype. (A) WB analysis of polarization-related protein expression in KCs, with quantitative analysis based on band intensity. (B) Immunohistochemical staining of CD86 and CD163. ns P > 0.05, *P < 0.05; **P < 0.01; ***P < 0.001; n = 6.

    Article Snippet: Comparisons between two groups were made using an unpaired Student’s t-test, Name Dilution Supplier Code GAPDH WB: 1:10000 Proteintech 60004-1-Ig Arg1 WB: 1:1000 Proteintech 16001-1-AP CD86 WB: 1:1000, IHC: 1:1000 Zenbio 380,350 P65 WB: 1:1000 Proteintech 66535-1-Ig p-P65 WB: 1:1000, IF: 1:500 CST 3033 IKKβ WB: 1:1000 ABclonal A2087 p-IKKβ WB: 1:1000 Proteintech A2087 IκBα WB: 1:1000 ABclonal A19714 p-IκBα WB: 1:1000 ABclonal AP0707 Goat anti-rabbit WB: 1:20000 Zenbio 550,018 CD68 IF: 1:100 Invitrogen MA5-13324 CD163 IHC: 1:1000 Proteintech 16646-1-AP ANGPTL4 IHC: 1:1000 Proteintech 18374-1-AP Table 2.

    Techniques: In Vivo, Expressing, Immunohistochemical staining, Staining

    Fig. 5. ANGPTL4 recombinant protein promotes M2 polarization of KCs. (A) RT-qPCR and ELISA were used to measure IL-10, IL-1β, and TNF-α expression levels in KCs isolated from rat liver and their corresponding levels in the cell supernatant. (B) WB analysis of polarization-related protein levels in KCs, with quantitative evaluation based on band intensity. (C) Flow cytometry was employed to assess M1-polarized KCs, using FITC-labeled CD68 for KC identification and APC-labeled CD86 to detect M1 polarization. ns P > 0.05, *P < 0.05; **P < 0.01; ***P < 0.001; n = 3.

    Journal: Scientific reports

    Article Title: ANGPTL4 induces Kupffer cell M2 polarization to mitigate acute rejection in liver transplantation.

    doi: 10.1038/s41598-024-81832-x

    Figure Lengend Snippet: Fig. 5. ANGPTL4 recombinant protein promotes M2 polarization of KCs. (A) RT-qPCR and ELISA were used to measure IL-10, IL-1β, and TNF-α expression levels in KCs isolated from rat liver and their corresponding levels in the cell supernatant. (B) WB analysis of polarization-related protein levels in KCs, with quantitative evaluation based on band intensity. (C) Flow cytometry was employed to assess M1-polarized KCs, using FITC-labeled CD68 for KC identification and APC-labeled CD86 to detect M1 polarization. ns P > 0.05, *P < 0.05; **P < 0.01; ***P < 0.001; n = 3.

    Article Snippet: Comparisons between two groups were made using an unpaired Student’s t-test, Name Dilution Supplier Code GAPDH WB: 1:10000 Proteintech 60004-1-Ig Arg1 WB: 1:1000 Proteintech 16001-1-AP CD86 WB: 1:1000, IHC: 1:1000 Zenbio 380,350 P65 WB: 1:1000 Proteintech 66535-1-Ig p-P65 WB: 1:1000, IF: 1:500 CST 3033 IKKβ WB: 1:1000 ABclonal A2087 p-IKKβ WB: 1:1000 Proteintech A2087 IκBα WB: 1:1000 ABclonal A19714 p-IκBα WB: 1:1000 ABclonal AP0707 Goat anti-rabbit WB: 1:20000 Zenbio 550,018 CD68 IF: 1:100 Invitrogen MA5-13324 CD163 IHC: 1:1000 Proteintech 16646-1-AP ANGPTL4 IHC: 1:1000 Proteintech 18374-1-AP Table 2.

    Techniques: Recombinant, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Expressing, Isolation, Flow Cytometry, Labeling

    Fig. 6. Hepatocyte-derived ANGPTL4 is crucial for suppressing inflammatory activation in KCs. (A) Schematic of the experimental procedure: co-culture of HCs with LPS-stimulated KCs. (B) ELISA analysis of ANGPTL4 levels in the KCs culture medium. (C) RT-qPCR and ELISA analysis of IL-10, IL-1β, and TNF-α expression levels. (D) WB analysis of polarization-related proteins in KCs, with quantitative assessment based on band intensity. (E) Flow cytometry analysis of M1-polarized cells, identifying KCs with FITC-labeled CD68 and M1 markers with APC-labeled CD86. (F) Western blot analysis of proteins related to the NF-κB pathway, with quantification based on band intensity. (G) Immunofluorescence staining to localize phosphorylated p65. ns P > 0.05, *P < 0.05; **P < 0.01; ***P < 0.001; n = 3.

    Journal: Scientific reports

    Article Title: ANGPTL4 induces Kupffer cell M2 polarization to mitigate acute rejection in liver transplantation.

    doi: 10.1038/s41598-024-81832-x

    Figure Lengend Snippet: Fig. 6. Hepatocyte-derived ANGPTL4 is crucial for suppressing inflammatory activation in KCs. (A) Schematic of the experimental procedure: co-culture of HCs with LPS-stimulated KCs. (B) ELISA analysis of ANGPTL4 levels in the KCs culture medium. (C) RT-qPCR and ELISA analysis of IL-10, IL-1β, and TNF-α expression levels. (D) WB analysis of polarization-related proteins in KCs, with quantitative assessment based on band intensity. (E) Flow cytometry analysis of M1-polarized cells, identifying KCs with FITC-labeled CD68 and M1 markers with APC-labeled CD86. (F) Western blot analysis of proteins related to the NF-κB pathway, with quantification based on band intensity. (G) Immunofluorescence staining to localize phosphorylated p65. ns P > 0.05, *P < 0.05; **P < 0.01; ***P < 0.001; n = 3.

    Article Snippet: Comparisons between two groups were made using an unpaired Student’s t-test, Name Dilution Supplier Code GAPDH WB: 1:10000 Proteintech 60004-1-Ig Arg1 WB: 1:1000 Proteintech 16001-1-AP CD86 WB: 1:1000, IHC: 1:1000 Zenbio 380,350 P65 WB: 1:1000 Proteintech 66535-1-Ig p-P65 WB: 1:1000, IF: 1:500 CST 3033 IKKβ WB: 1:1000 ABclonal A2087 p-IKKβ WB: 1:1000 Proteintech A2087 IκBα WB: 1:1000 ABclonal A19714 p-IκBα WB: 1:1000 ABclonal AP0707 Goat anti-rabbit WB: 1:20000 Zenbio 550,018 CD68 IF: 1:100 Invitrogen MA5-13324 CD163 IHC: 1:1000 Proteintech 16646-1-AP ANGPTL4 IHC: 1:1000 Proteintech 18374-1-AP Table 2.

    Techniques: Derivative Assay, Activation Assay, Co-Culture Assay, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Expressing, Flow Cytometry, Labeling, Western Blot, Immunofluorescence, Staining

    Fig. 7. Knockdown of ANGPTL4 expression in HCs within the co-culture system promotes M1 polarization of KCs. (A) Schematic representation of the experimental procedure: siRNA-mediated knockdown of ANGPTL4 in HCs co-cultured with LPS-stimulated KCs. (B) RT-qPCR and ELISA assessments of IL-10, IL-1β, and TNF-α levels. (C) WB analysis of polarization-related proteins in KCs, with quantification of band intensity. (D) Flow cytometry of M1-type polarized cells, with FITC-labeled CD68 and APC-labeled CD86. (E) Western blot assessment of proteins associated with the NF-κB pathway, including band intensity quantification. (F) Immunofluorescence staining to identify the localization of phosphorylated p65. ns P > 0.05, *P < 0.05; **P < 0.01; ***P < 0.001; n = 3.

    Journal: Scientific reports

    Article Title: ANGPTL4 induces Kupffer cell M2 polarization to mitigate acute rejection in liver transplantation.

    doi: 10.1038/s41598-024-81832-x

    Figure Lengend Snippet: Fig. 7. Knockdown of ANGPTL4 expression in HCs within the co-culture system promotes M1 polarization of KCs. (A) Schematic representation of the experimental procedure: siRNA-mediated knockdown of ANGPTL4 in HCs co-cultured with LPS-stimulated KCs. (B) RT-qPCR and ELISA assessments of IL-10, IL-1β, and TNF-α levels. (C) WB analysis of polarization-related proteins in KCs, with quantification of band intensity. (D) Flow cytometry of M1-type polarized cells, with FITC-labeled CD68 and APC-labeled CD86. (E) Western blot assessment of proteins associated with the NF-κB pathway, including band intensity quantification. (F) Immunofluorescence staining to identify the localization of phosphorylated p65. ns P > 0.05, *P < 0.05; **P < 0.01; ***P < 0.001; n = 3.

    Article Snippet: Comparisons between two groups were made using an unpaired Student’s t-test, Name Dilution Supplier Code GAPDH WB: 1:10000 Proteintech 60004-1-Ig Arg1 WB: 1:1000 Proteintech 16001-1-AP CD86 WB: 1:1000, IHC: 1:1000 Zenbio 380,350 P65 WB: 1:1000 Proteintech 66535-1-Ig p-P65 WB: 1:1000, IF: 1:500 CST 3033 IKKβ WB: 1:1000 ABclonal A2087 p-IKKβ WB: 1:1000 Proteintech A2087 IκBα WB: 1:1000 ABclonal A19714 p-IκBα WB: 1:1000 ABclonal AP0707 Goat anti-rabbit WB: 1:20000 Zenbio 550,018 CD68 IF: 1:100 Invitrogen MA5-13324 CD163 IHC: 1:1000 Proteintech 16646-1-AP ANGPTL4 IHC: 1:1000 Proteintech 18374-1-AP Table 2.

    Techniques: Knockdown, Expressing, Co-Culture Assay, Cell Culture, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Labeling, Western Blot, Immunofluorescence, Staining